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- RPI M42560-500.0 MacConkey Agar, 500g
RPI M42560-500.0 MacConkey Agar, 500g
NZD 332
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Good to Excellent results for multiple key microorganisms.
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Product Details
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Microbiology Laboratories
Ideal for testing and isolating gram-negative bacteria through its selective properties and nutrient availability.
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Educational Institutions
Suitable for teaching students about microbial growth, coliforms, and lactose fermentation in controlled experiments.
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Clinical Diagnostics
Perfect for laboratories analyzing stool samples to identify enteric pathogens, ensuring accurate diagnostic results.
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Non-professional Use
Not suitable for individuals without microbiology training, as improper use can lead to inaccurate results.
Product Description
RPI M42560-500.0 MacConkey Agar, 500g
Customer Questions & Answers
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Question:
What is MacConkey Agar used for?
Answer: MacConkey Agar is a selective and differential culture medium that is primarily used for the isolation and differentiation of gram-negative bacteria. It contains bile salts and crystal violet, which inhibit the growth of gram-positive organisms, making it ideal for isolating enteric bacteria. It is highly useful in clinical microbiology laboratories for diagnosing infections caused by pathogens like E. coli and Salmonella. -
Question:
How do you prepare MacConkey Agar?
Answer: To prepare MacConkey Agar, you need to dissolve the agar powder in distilled water according to the manufacturer's instructions. Heat the solution to boiling to ensure complete dissolution, then autoclave it to sterilize. After cooling to around 45-50°C, pour it into sterile Petri dishes. This preparation method ensures that the medium is free from contamination and ready for microbial culture. -
Question:
What should I look for when choosing MacConkey Agar?
Answer: When selecting MacConkey Agar, consider its quality, formulation, and the specific application you have in mind. Ensure it has the correct balance of nutrients to support the growth of desired gram-negative bacteria while inhibiting gram-positive strains. Additionally, look for certifications or compliance with industry standards, which guarantees reliability for laboratory use, especially in clinical or research settings. -
Question:
What types of bacteria can be cultured on MacConkey Agar?
Answer: MacConkey Agar is particularly effective for culturing enteric bacteria such as Escherichia coli, Salmonella, Shigella, and Klebsiella. The medium not only supports their growth but also allows differentiation based on lactose fermentation, indicated by color changes in colony appearance. This makes it a vital tool in identifying bacterial pathogens responsible for gastrointestinal infections. -
Question:
Can MacConkey Agar be used for testing antibiotic susceptibility?
Answer: While MacConkey Agar is primarily used for isolating and identifying gram-negative bacteria, it can support preliminary antibiotic susceptibility testing. However, for accurate results, specialized media and methods should be employed. Using MacConkey Agar can help identify resistant strains during the initial stages of culture, facilitating timely therapeutic interventions. -
Question:
How is colony morphology interpreted on MacConkey Agar?
Answer: Colony morphology on MacConkey Agar can provide insights into the type of bacteria present. For instance, lactose fermenters like E. coli produce pink colonies due to the acid produced during fermentation, while non-fermenters such as Salmonella can produce colorless colonies. By examining the growth characteristics and coloration, microbiologists can effectively narrow down the identity of the bacteria cultured. -
Question:
How long can prepared MacConkey Agar plates be stored?
Answer: Prepared MacConkey Agar plates can generally be stored in a cool, dark place for a limited time, typically a few weeks. It is crucial to ensure they remain sealed to prevent contamination. However, for optimal performance and accurate results, it's advisable to use freshly prepared plates whenever possible, especially in diagnostic settings where precision is critical. -
Question:
Is there a specific temperature for incubating MacConkey Agar?
Answer: The ideal incubation temperature for MacConkey Agar is 35-37°C, which is optimal for the growth of most gram-negative bacteria. Incubation at this temperature for 18-24 hours allows for effective growth and differentiation of lactose fermenters from non-fermenters. Adhering to the correct temperature range is crucial for obtaining reliable results in microbiological analysis. -
Question:
What is the shelf life of RPI M42560-500.0 MacConkey Agar?
Answer: The shelf life of RPI M42560-500.0 MacConkey Agar is typically indicated on the packaging. Generally, if stored under proper conditions—away from light and moisture—it can last for an extended period. Always check the expiry date provided by the manufacturer to ensure the integrity and effectiveness of the agar before use. -
Question:
Where can I buy RPI M42560-500.0 MacConkey Agar, 500g?
Answer: You can purchase RPI M42560-500.0 MacConkey Agar, 500g through Ubuy in New Zealand. Ubuy provides a hassle-free purchasing experience and often has a range of laboratory supplies and reagents, ensuring you can find what you need for your microbiological applications.
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NZD 332
Order now and get it around Friday, August 28
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Features & Benefits
- Free flowing beige or pale pink appearance.
- Effective against key microorganisms, achieving 'Good to Excellent' ratings.
- pH level of 6.9 - 7.3 in a 5.0% solution.
- Colorless results for harmful strains like E. Coli and Salmonella.
- Ideal for safe and hygienic applications.
- Proven efficacy ensures peace of mind for users.
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