What is the purpose of Accuris Instruments PR2000-N-500, Green Real Time PCR Mix?

The Accuris Instruments PR2000-N-500, Green Real Time PCR Mix is designed for use in quantitative PCR applications. It is formulated to provide high sensitivity and specificity in amplifying DNA, making it ideal for research laboratories and diagnostic applications. Its unique green dye enables real-time monitoring of PCR reactions, simplifying the process of data analysis and interpretation. For laboratories focused on gene expression studies, pathogen detection, or genetic variation analysis, this mix offers reliable performance and reproducibility.

Can the PR2000-N-500 mix be used for both DNA and RNA assays?

Yes, the PR2000-N-500 mix can be utilized for both DNA and RNA assays. When utilizing it for RNA, it is recommended to include a reverse transcription step to convert RNA into complementary DNA (cDNA) prior to the PCR amplification. This versatility makes the mix suitable for a wide range of applications, including quantifying gene expression and detecting viral RNA in clinical samples. It provides researchers with the flexibility needed to conduct diverse real-time PCR analyses.

What makes the Green Real Time PCR Mix different from traditional PCR mixes?

The Green Real Time PCR Mix includes a fluorescent dye that enables simultaneous observation of the PCR process in real time. Unlike traditional PCR mixes, which require post-amplification analysis, the green dye allows for immediate visualization and quantification during the PCR cycles. This feature streamlines experimental procedures, saves time, and enhances accuracy in quantifying target DNA. Researchers often find this mix indispensable for high-throughput analyses and when rapid results are essential.

What is the optimal storage condition for Accuris Instruments PCR Mix?

The Accuris Instruments PR2000-N-500 PCR Mix should be stored at -20°C in a non-frost-free freezer to maintain its stability and performance. Proper storage ensures that the enzymes and reagents remain active, allowing for reliable results during PCR amplifications. When needed, the mix can be aliquoted to prevent repeated freeze-thaw cycles, which can degrade the product. Utilizing optimal storage techniques is crucial for laboratories that rely on consistent and dependable results from their PCR experiments.

How can the PR2000-N-500 improve PCR efficiency?

The Accuris Instruments PR2000-N-500 Mix is formulated with components that enhance the efficiency of the PCR process. It contains optimized concentrations of buffer, salts, and DNA polymerase that facilitate robust amplification, resulting in higher yield and specificity of target sequences. Laboratories focusing on demanding applications, such as multi-target amplification or detecting low-abundance targets, will benefit from this improved efficiency. Utilizing this mix can also lead to reduced cycle times, enhancing throughput for high-demand research applications.

Is this mix suitable for high-throughput screening?

Yes, the PR2000-N-500 mix is suitable for high-throughput screening applications due to its reliable performance and consistent results across multiple samples. Its formulation enables rapid cycling conditions, which enhances throughput without compromising data quality. This makes it ideal for laboratories involved in large-scale genetic screening, diagnostic testing, or environmental analyses. With the capability to analyze numerous samples efficiently, researchers can maximize their productivity and expedite research timelines.

What is the recommended primer design for optimal results with this mix?

For optimal results when using the PR2000-N-500 mix, it is recommended to design primers that are specific to the target sequences, with a melting temperature (Tm) of around 58-60°C. It's essential to ensure that primers are free of secondary structures and have a balanced GC content to promote efficient amplification. This meticulous primer design can significantly enhance the specificity and yield of PCR products. Laboratories can additionally benefit from using software tools to assist in the design process, ensuring robust experimental outcomes.

Can I use this product for multiplex PCR?

Yes, the Accuris Instruments PR2000-N-500 mix can be used for multiplex PCR applications, allowing simultaneous amplification of multiple DNA targets in a single reaction. To achieve optimal results, it's essential to carefully design primers to ensure compatibility and specificity for all targets involved. Proper optimization of reaction conditions, such as adjusting primer concentrations and annealing temperatures, will enhance multiplex efficiency. This capability is particularly useful in applications such as pathogen detection or genetic polymorphism analysis, where time and sample volumes are limited.

How do I troubleshoot common PCR issues with this mixture?

Common PCR issues such as non-specific amplification, low yield, or no amplification can be addressed by optimizing various parameters when using the PR2000-N-500 mix. Start with primer redesign to improve specificity, check that reaction components are mixed thoroughly, and adjust the annealing temperature if necessary. Additionally, consider optimizing template DNA concentration. Keeping a detailed experimental log will help in pinpointing issues and refining the protocol. This troubleshooting approach is essential for achieving accurate and reproducible results.

Where can I buy Accuris Instruments PR2000-N-500, Green Real Time PCR Mix, 5 ml?

You can purchase the Accuris Instruments PR2000-N-500, Green Real Time PCR Mix, 5 ml through Ubuy in New Zealand. Ubuy offers a reliable platform for acquiring laboratory supplies, including this advanced PCR mix, ensuring you have access to quality products tailored for your research needs.

How to Shop Instruments PR2000-N-500, Green Real Time PCR Mix, 5 Online From Ubuy?

It’s easy to shop Instruments PR2000-N-500, Green Real Time PCR Mix, 5 online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.

Is Instruments PR2000-N-500, Green Real Time PCR Mix, 5 Available to Shop Online in New Zealand?

Yes, at Ubuy New Zealand this product is available for you to shop at a reasonable price. The Instruments PR2000-N-500, Green Real Time PCR Mix, 5 is not available locally but you can trust us with our express shipping services.

How Long Does It Take to Get Product After Placing the Order?

The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

Accuris

Instruments PR2000-N-500, Green Real Time PCR Mix, 5 ml

Superior sensitivity and fast cycling with exceptional results.

Accuris

Item #: 78610455

Instruments PR2000-N-500, Green Real Time PCR Mix, 5 ml

82% of respondents would recommend this to a friend

Item #: 78610455

NZD 1003

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What Stands Out

High Sensitivity

Delivers exceptional precision in quantifying nucleic acids, ensuring reliable results even with low template concentrations, making it ideal for challenging samples.

Fast Amplification

Enables rapid PCR cycling times, significantly reducing overall experiment duration while maintaining high specificity and sensitivity, enhancing laboratory efficiency.

Versatile Application

Suitable for a wide range of applications, from gene expression analysis to pathogen detection, catering to diverse research and diagnostic needs across various scientific fields.

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0.5 lbs (230 grams)

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Molecular Biologists
Ideal for researchers needing precise and reliable PCR results in various applications, including gene expression analysis.
Academic Labs
Perfect for educational institutions conducting experiments that require real-time PCR technology with consistent performance.
Diagnostic Laboratories
Useful for laboratories specializing in genetic testing and pathogen detection, offering high sensitivity and specificity.

Beginner Users
Not suitable for those unfamiliar with PCR techniques who may struggle with the requirements of real-time PCR.

Product Description

Instruments PR2000-N-500, Green Real Time PCR Mix, 5 ml

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Question: How to Shop Instruments PR2000-N-500, Green Real Time PCR Mix, 5 Online From Ubuy?

Answer: It’s easy to shop Instruments PR2000-N-500, Green Real Time PCR Mix, 5 online from Ubuy. You just have to search for the product, choose your shipping method while checking out and get it delivered to your location.
Question: Is Instruments PR2000-N-500, Green Real Time PCR Mix, 5 Available to Shop Online in New Zealand?

Answer: Yes, at Ubuy New Zealand this product is available for you to shop at a reasonable price. The Instruments PR2000-N-500, Green Real Time PCR Mix, 5 is not available locally but you can trust us with our express shipping services.
Question: How Long Does It Take to Get Product After Placing the Order?

Answer: The delivery time of your ordered product varies as per what you've ordered and the shipping method that you've chosen. The estimated delivery time is mentioned during the checkout process, so be carefree while shopping.

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High sensitivity and fast cycling
Ideal for low copy number templates
Early Ct values and detection across a broad dynamic range
Includes Accuris Hot Start Taq Polymerase for greater specificity and accuracy
Ready to use 2x mastermix

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Instruments PR2000-N-500, Green Real Time PCR Mix, 5 ml

NZD 1003

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