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Item #:
223170807
DMEM Cell Culture Medium/High Glucose (1)
Item #:
223170807
NZD 60
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DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle’s Minimal Essential Medium.
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Product Details
| % Assay of Chemical(s) | 10 |
| Container Type | Bottle |
| Item Form | Liquid |
| Volume | 500 ml |
| Brand Name | Hygia Reagents |
| Unit Count | 1 Count |
| Manufacturer | Hygia Reagents |
| UPC | 749799141579 |
| Included Components | 1 bottle |
| Part Number | HCM-DMEM-LG |
| Brand | Hygia Reagents |
| Item Volume | 500 ml |
| Percentage assay | 10 |
| Package Information | Bottle |
| Item Weight | 1 lbs (450 grams) |
Product Description
DMEM Cell Culture Medium/High Glucose (1)
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Customer Questions & Answers
-
Question:
What is DMEM Cell Culture Medium/High Glucose used for?
Answer: DMEM (Dulbecco's Modified Eagle Medium) Cell Culture Medium/High Glucose is primarily used for culturing various mammalian cells in a laboratory setting. The high glucose concentration makes it suitable for cell types that require increased energy, such as fast-growing cells like fibroblasts and some cancer cell lines. It's designed to support optimal growth and maintenance of cells by providing essential nutrients, vitamins, and amino acids. -
Question:
How do I prepare DMEM Cell Culture Medium/High Glucose?
Answer: To prepare DMEM Cell Culture Medium/High Glucose, dilute the powder form in distilled water according to the manufacturer's instructions, typically mixing with a sterile buffer solution. Ensure that you adjust the pH of the medium to a physiological level (around 7.2-7.4) as this is critical for successful cell culture. When using this medium, having sterile techniques is paramount to prevent contamination, ensuring reliable and reproducible results in your experiments. -
Question:
Can I use DMEM Cell Culture Medium/High Glucose for all types of cells?
Answer: While DMEM Cell Culture Medium/High Glucose supports a wide range of cell types, it's not universally suitable for all cells. Some specific cell lines may require specialized media that better meet their nutritional needs, or may prefer a lower glucose concentration. For instance, certain primary cells or sensitive cell lines may thrive better in a different formulation. It's important to assess the requirements of your specific cell line before making a selection. -
Question:
Is DMEM Cell Culture Medium/High Glucose suitable for serum-free culture?
Answer: DMEM Cell Culture Medium/High Glucose can be tailored for serum-free cultures but usually contains serum supplements under standard conditions. Serum provides growth factors crucial for various cell types. If you're working in serum-free conditions, consider adding specific supplements that mimic the effects of serum, adjusting the medium appropriately to fulfill the cell's biological requirements for optimal growth and survival. -
Question:
What conditions should I store DMEM Cell Culture Medium/High Glucose?
Answer: DMEM Cell Culture Medium/High Glucose should be stored in a cool, dry place at temperatures recommended by the manufacturer, typically at 2-8°C for liquid forms or at room temperature for powdered forms. Avoid exposure to direct sunlight and extreme temperatures, as these can degrade the medium's components. Proper storage ensures that the medium remains effective for your cell culture applications and maintains its nutrient stability. -
Question:
How does DMEM Cell Culture Medium/High Glucose differ from other cell culture media?
Answer: DMEM Cell Culture Medium/High Glucose differs from other media formulations primarily in its higher glucose content and additional vitamins and amino acids. This formulation supports not just basic cell survival but also enhances proliferation and metabolism in fast-growing cell lines. It's specifically designed for cells requiring high glucose to fuel their growth compared to media like RPMI or F-10, which have varied formulations to cater to different cellular needs. -
Question:
What are the benefits of using DMEM Cell Culture Medium/High Glucose?
Answer: The key benefits of using DMEM Cell Culture Medium/High Glucose include its ability to support a variety of cell lines due to its rich nutrient composition. High glucose levels enable robust energy metabolism, essential for proliferating cells. Additionally, it helps maintain optimal physiological conditions for cells, contributing to reproducibility in experiments. Researchers often favor DMEM when engaging in cell proliferation studies, drug testing, or gene expression research. -
Question:
Can I freeze DMEM Cell Culture Medium/High Glucose for future use?
Answer: Freezing DMEM Cell Culture Medium/High Glucose is generally not recommended, especially for liquid forms, as it can lead to crystallization and degradation of components. However, some labs might freeze aliquots for specific short-term uses. If you choose to freeze it, ensure that it's in a suitable container and consider the impact on cell viability once thawed, as piecing apart the medium can lead to changes in nutrient availability. -
Question:
How can I determine if my DMEM Cell Culture Medium/High Glucose is still usable?
Answer: To determine the usability of DMEM Cell Culture Medium/High Glucose, check for any visible changes such as discoloration, cloudiness, or precipitation. Additionally, you can assess its pH level, which should be around 7.2 to 7.4. Always refer to the expiration date provided by the manufacturer. If you've opened the bottle, ensure that it has been stored correctly with minimal contamination to retain its integrity for cell culture. -
Question:
Where can I buy DMEM Cell Culture Medium/High Glucose in New Zealand?
Answer: You can buy DMEM Cell Culture Medium/High Glucose at Ubuy. They offer a wide range of laboratory supplies and are known for their reliable service and product availability. Whether you're looking for bulk quantities or smaller packs, Ubuy provides various options to suit your needs, making it convenient to ensure you have the right cell culture medium for your research.
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Pros
- Exceptional quality
- User-friendly design
- Great performance
- Highly recommended by experts
- Long-lasting durability
Cons
- Limited color options.
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NZD 60
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Features & Benefits
- Supports the growth of various mammalian cells.
- Contains 4x concentration of amino acids and vitamins.
- Available with low or high glucose levels.
- Includes phenol red as a pH indicator.
- Manufactured under strict quality control at a cGMP-compliant facility.
- Suitable for multiple cell types and requires supplementation.
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